Shear-enhanced binding of intestinal colonization factor antigen I of enterotoxigenicEscherichia coli
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چکیده
منابع مشابه
Shear-enhanced binding of intestinal colonization factor antigen I of enterotoxigenic Escherichia coli.
In the intestine, enterotoxigenic Escherichia coli works against peristaltic forces, adhering to the epithelium via the colonization factor antigen I (CFA/I) fimbrial adhesin CfaE. The CfaE adhesin is similar in localization and tertiary (but not primary) structure to FimH, the type 1 fimbrial adhesin of uropathogenic E. coli, which shows shear-dependent binding to epithelial receptors by an al...
متن کاملThe major subunit, CfaB, of colonization factor antigen i from enterotoxigenic Escherichia coli is a glycosphingolipid binding protein.
Bacterial adherence to mucosal surfaces is an important virulence trait of pathogenic bacteria. Adhesion of enterotoxigenic Escherichia coli (ETEC) to the intestine is mediated by a number of antigenically distinct colonization factors (CFs). One of the most common CFs is CFA/I. This has a fimbrial structure composed of a major repeating subunit, CfaB, and a single tip subunit, CfaE. The potent...
متن کاملIdentification of Novel Components Influencing Colonization Factor Antigen I Expression in Enterotoxigenic Escherichia coli
Colonization factors (CFs) mediate early adhesion of Enterotoxigenic Escherichia coli (ETEC) in the small intestine. Environmental signals including bile, glucose, and contact with epithelial cells have previously been shown to modulate CF expression in a strain dependent manner. To identify novel components modulating CF surface expression, 20 components relevant to the intestinal environment ...
متن کاملMonoclonal antibodies against different epitopes on colonization factor antigen I of enterotoxin-producing Escherichia coli.
Hybridoma-secreting monoclonal antibodies (MAbs) against colonization factor antigen I (CFA/I) were produced by the fusion of spleen cells from immunized BALB/c mice with F/O myeloma cells. The 25 MAbs with the highest antibody titer against CFA/I, as determined by an enzyme-linked immunosorbent assay, were studied for specificity and the capacity to agglutinate CFA/I-carrying bacteria. Most of...
متن کاملIsopycnic separation of Escherichia coli cultures possessing colonization factor antigen I.
A culture of Escherichia coli possessing colonization factor antigen I was subjected to isopycnic separation on Percoll gradients. The results demonstrated successful division of the culture into two populations: (i) bacteria which cause mannose-resistant hemagglutination and (ii) bacteria which lack the ability to hemagglutinate in the presence of mannose.
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2010
ISSN: 0950-382X,1365-2958
DOI: 10.1111/j.1365-2958.2010.07116.x